[ Links ], BROWN, B. J.; MITCHELL, R. J.; GRAHAM, S. Competition for pollination between an invasive species (purple loosestrife) and a native congener. 1). 2007). Part of Springer Nature. 04:30h) to afternoon (approx. Undamaged, fully developed flowers were dried in a VirTis AdVantage Plus tray lyophilizer (Gardiner, USA). https://doi.org/10.1016/j.fct.2018.12.024, Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A, Bray F (2019) Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. 1 and two Halictidae bees in Botucatu and Melitoma segmentaria, Ancyloscelis apiformis, Exomalopsis fulvofasciata and Trigona spinipes in Jaboticabal. - 126.96.36.199. 4). Ann. Consequently, further analysis with accurate and relevant tests such as the mouse local lymph node assay (LLNA) and human the cell activation test (h-CLAT) are required to investigate the presence of chemical sensitizers in IHFE. https://doi.org/10.1016/j.jphotobiol.2015.02.024, Delgado GC, Buril MT, Alves M (2014) Convolvulaceae do Parque Nacional do Catimbau, Pernambuco, Brasil. [ Links ], NEWSTROM, L. E.; FRANKIE, G. W.; BAKER, H. G. A new classification for plant phenology based on flowering patterns in lowland tropical rain forest trees at La Selva, Costa Rica. Some studies have shown the protective effect of phenolic compounds detected in IHFE, on the skin carcinogenesis induced by UV. 2019). Oxford: Oxford University Press, 1992. p. 32-38. According to Gentry (1974a), Bignoniaceae species that flower strongly over a period of 38 weeks have these ephemeral flowers and are visited by a wide range of bees, butterflies, hummingbirds and hawkmoths. Jaboticabal climate is humid, subtropical with dry winter or Koeppen's Cwa (Aloisi & Demattê, 1974). Com relação aos visitantes florais, a análise de agrupamento, empregando-se o índice de similaridade de Jaccard, indicou maior similaridade entre diferentes espécies de Ipomoea ocorrentes no mesmo local do que entre populações da mesma espécie em diferentes localidades. 2012). Ipomoea cairica bloomed all year round, in a phenological pattern similar to that defined as continual by Newstrom et al. https://doi.org/10.1016/S0031-9422(03)00273-5, Zhang L, Mu X, Fu J, Zhou Z (2007) In vitro cytotoxicity assay with selected chemicals using human cells to predict target-organ toxicity of liver and kidney. Ipomoea horsfalliae Hook., Convolvulaceae, flowers were collected from an experimental plot at CENIVAM (N 07° 08,422′ W 073° 06,960′) in March 2017. For each visitor, abundance was registered considering the number of recorded visits. The promiscuous and opportunistic features of the flowers were shown, with such type of adaptation to pollination being advantageous to weeds since pollinator availability is unpredictable at ruderal environments. Plant in moist, humus rich, well drained soil and keep a regular watering schedule throughout the year. O caráter promíscuo e oportunista da adaptação à polinização, presente nessas espécies, foi demonstrado, sendo essa adaptação vantajosa para plantas daninhas, uma vez que em ambientes ruderais a disponibilidade de polinizadores é imprevisível. 3.ed. [ Links ], KEVAN, P. G. Vegetation and floral colors revealed by ultraviolet light: interpretational difficulties for functional significance. Calibration Strategy 2 resulted in younger divergence time estimates (Fig. https://doi.org/10.1016/j.ijms.2003.10.008, Meira M, Silva EP, David JM, David JP (2012) Review of the genus Ipomoea: traditional uses, chemistry and biological activities. Ultraviolet (UV) radiation can cause cancer, premature aging, sunburns, and wrinkles. Figure 3 illustrates the extent of histological skin lesions of BALB/c mice from group B, treated with ethanol (70% v/v) and UV-B. San Francisco: W. H. Freeman, 1973. Uses Containers, Flower Beds, Hanging Baskets Care. 14:00h), during several days along the study years. Ipomoea spp. When THP-1 cells were stimulated with the representative sensitizer, 2-aminophenol, the level of IL-8 in the culture medium increased (from 1.079 ± 44 to 247 ± 77 pg/ml in non-stimulated cells; p < 0.01, ANOVA). Groups B and D were irradiated for 3 min with a Spectroline EB-280C UV-B lamp (λ = 312 nm), located 15 cm away, with an irradiation dose of 6 mJ/cm2. Here we report on the isolation of novel complementary and genomic DNA from a model for a short-day plant, Ipomoea nil (Japanese morning glory), that encodes 66- or 76-amino acid residue proteins. The visitors were collected and identified. 2, mass spectra of kaempferol-diglucoside and pelargonidin-sophoroside-glucoside are shown. Matrix True Blue Pansy has clear, purplish blue flowers. Previous experiments indicate that the white‐flowered a allele has a transmission advantage because of increased selfing and no detectable pollen discounting. 2089-2094 e-ISSN:2581-6063 (online), ISSN:0972-5210 POLLEN GRAINS TAXONOMY MATCHES DNA-BARCODING TAXONOMY IN DISCRIMINATION BETWEEN SOME CONVOLVULACEAE TAXA Hany S. Abd El-Raouf 1,2, Mohamed Helmy 3*, Mohamed Awad 4 and Ahmed M. El-Taher 1 1 Agricultural Botany Department, Faculty of Agriculture, Al-Azhar University, … It was not cytotoxic for both cancerous and normal cells, and no sensitizing effect was observed. EQ and REO contributed to evaluating cytotoxic and sensitizing activities. The species were analyzed for reproductive phenology, floral traits (shape, color, texture, size, nectar and scent), floral longevity, number of flowers per inflorescence, breeding systems and pollination ecology. https://doi.org/10.1248/bpb.23.23, Kwak CS, Yang J, Shin CY, Chung JH (2018) Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin. Photoprotective activity was measured, according to Estrella-Parra et al. Floral biology and breeding system of three Ipomoea weeds, Biologia floral e sistema reprodutivo de três espécies daninhas de Ipomoea. Flow was 0.3 ml/min and the injection volume 1 μl. I.horsfalliae flower extract appears to be a good starting point for research programs leading to the development of natural skin care products. 1986), and antidiabetic properties (Kusano and Abe 2000). The non-specificity of these three weeds in relation to pollination is advantageous, considering that, at ruderal environments, pollinator availability is unpredictable (Baker, 1965; 1974); this adaptation is frequently associated to plants living in anthropic, disturbed environments (Valentine, 1978), such as the sites studied in this work. Cientifica, v. 2, p. 123-136, 1974. [ Links ], MAIMONI-RODELLA, R. C. S. et al. Am. Ipomoea is a very important genus in this family, constituting of many weeds, some garden ornamentals and edible species. [ Links ], BAKER, H. G. Characteristics and modes of origin of weeds. Essential Ipomoea floral traits (Table 2, Figure 2) reveal that several of them are characteristics of melittophily, as described by Faegri & Pijl (1979). In Fig. The visitors were considered legitimate pollinators or robbers, based mainly on behavior and also on RA values. Flowers of these Ipomoea were visited by several species of bees, butterflies, beetles, one Tabanidae and one Scoliidae (Table 4). https://doi.org/10.1016/j.jep.2018.05.008, Lu TS, Saito N, Yokoi M, Shigihara A, Honda T (1992) Acylated pelargonidin glycosides in the red-purple flowers of Pharbitis nil. Photochem Photobiol 86:1005–1007. Sweet Potato Vines are a wonderful addition to gardens and containers, with their rich, heart-shaped leaves and trailing growth. The Convolvulaceae contain species adapted to several pollination syndromes (Vogel, 1954), such as melittophily, which seems to be rather frequent. The species were tested for spontaneous self-pollination according to Dafni (1992). Samples of 5 μm thickness were cut in a Leica® RM2125RT rotation microtome (Leica Biosystems, Wetzlar, Germany). This last consideration needs further investigation. Anthesis started around 05:00h and lasted mostly until afternoon (Table 2), when the corollas wilted and fell off. The mobile phase was as follows: A: water (0.2% formic acid) and B: acetonitrile (0.2% formic acid). 30, 69–79 (2020). https://doi.org/10.1111/j.1751-1097.2010.00776.x, Takahashi T, Kimura Y, Saito R, Nakajima Y, Ohmiya Y, Yamasaki K, Aiba S (2011) An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. SC, stratum corneum; E, epidermis; BC, burn cells; Ht, hypertrophy; At, atrophy; Pl, pleomorphism; D, dermis; SG, sebaceous gland; HF, hair follicle; RF, reactive fibroblasts; BV, blood vessel; M, muscle; IF, inflammatory infiltrates; H, hypodermis. Res. Some morning glories, such as Ipomoea muricata, are night blooming flowers. Biotropica, v. 6, p. 64-68. Non-treated cells were run in parallel. Dicaffeoylquinic acid, chlorogenic acid, … Phytochemistry 31:289–295. 418 p. [ Links ], RADFORD, A. E. et al. At the opening time, for all species, the stigma was receptive and the anthers were dehisced. Nevertheless, the result does not exclude the potential effect on other cancer cells. However, I. cairica and I. grandifolia had flowering peaks occurring at different periods of the year. Toxicol In Vitro 30:318–324. 3). 20, Supplement 2, 2020 pp. Cluster analysis using Jacquard similarity index indicated a greater similarity among different plant species in the same locality than among the populations at different places, in relation to floral visitor sets. There are two sweetpotato germplasm collections (Plant Genetic Resources Conservation Unit and US Vegetable Laboratory) maintained by the USDA, ARS for sweetpotato crop improvement. MTT assay (Mosmann 1983) was used to evaluate the potential of IHFE to reduce the viability of human cells. The data obtained was processed with the Thermo XCalibur™ Roadmap software, version 188.8.131.52. 2018), and vasodilator (Kwon et al. For XP cells, CC50 values were lower compared with normal cells (XP4PA, 110 ± 20 μg/ml; XP12RO-SV, 63 ± 5 μg/ml), which was expected since the cells were deficient in a key DNA repair protein, and therefore, they were more sensitive to treatment with IHFE (Maher et al. Brasil. The mixture was filtered, and the residue was extracted twice more. See more ideas about flower seeds, seeds, flowers. Detailed descriptions of these Ipomoea weeds were provided by Carrizo & Sobrero (2001). The fragmentation pattern of these compounds is characterized by the consecutive loss of sugar moieties from [M + H]+ ions. In the epidermis, round burn cells were detected, with contracted nuclei and eosinophilic cytoplasm; the extension of these cells was multifocal and smaller than that of the group without protective substance. 2002) activities. PubMed Biol Pharm Bull 23:23–26. Phytochemistry 63:721–726. (Ed.). In 2018, MC caused 518 deaths and 1907 new cases were registered (The Global Cancer Observatory 2019). have applications in medicine, due to their hypotensive, antimicrobial, anticancer (Bieber et al. 2-Aminophenol is recognized as a strong sensitizer (Parise et al. 700 species distributed in tropical and subtropical regions (Meira et al. Parsol® was purchased from Alfadelta S.A. of C.V. (Naucalpan de Juárez, Mexico). are annual herbs with sexual reproduction only (Lorenzi, 2000). Mo. 101, June 3, 2014, Colombian Ministry of Environment and Sustainable Development). Phytochemistry 25:1077–1081. New York: Academic Press, 1965. p. 147-172. Cambridge: Cambridge University Press, 1994. p. 105-121. https://doi.org/10.1016/0022-1759(83)90303-4, Moon PD, Lee BH, Jeong HJ, An HJ, Park, SJ, Kim HR, Ko SG, Um JY, Hong SH, Kim HM (2007) Use of scopoletin to inhibit the production of inflammatory cytokines through inhibition of the IĸB/NF-ĸB signal cascade in the human mast cell line HMC-1. Revista Brasileira de Farmacognosia The pollen/ovule ratios indicate that I. cairica is alogamous and that I. grandifolia and I. nil are facultative autogamous, according to Cruden (1977). A standardized reference value of effective dose 50 in the THP-1/IL-8 assay is not available to estimate the sensitizing effect of plant extracts. This procedure was repeated three times, every 24 h. All mice were sacrificed by asphyxia in a CO2 chamber, 48 h after receiving their last radiation dose. Extension of lesions evaluated in the skin of the BALB/c mice treated with ethanol (70%, v/v), Parsol®, or Ipomea horsfalliae flower extract (3 mg/ml), exposed to UV-B. Ipomea horsfalliae flower extract chromatogram (extracted ion chromatogram, EIC), obtained by UHPLC-ESI+-Orbitrap-MS. 1974b [ Links ], INOUYE, D. W. The terminology of floral larceny. [ Links ], GENTRY, A. H. Co evolutionary patterns in Central American Bignoniaceae. Turrialba, v. 41, p. 344-349, 1991. Coumarin derivatives reduced the embryotoxic effects of UV-B radiation in sea urchin gametes and have been considered as possible photoprotectors (de Araujo Leite et al. All procedures were carried out in accordance with Official Mexican Regulation (NOM-062-ZOO-1999). We thank Dr. João M. F. Camargo for the identification of the bees collected in Jaboticabal and Drs. Skin cancer is a malignant tumor disease common in fair-skinned, light-eyed people, with blond hair (Ferlay et al. International Research Journal of Biological Sciences _____ ISSN 2278-3202 Vol. Ipomoea grandifolia and I. nil. and E. nigrita, in Jaboticabal, as the most effective pollinators. Three independent experiments in duplicate were carried out. The browser also provides tracks displaying evide… New York: John Wiley & Sons, 1988. In relation to I. grandifolia, the bees Apis mellifera, Ancyloscelis sp., Diadasina riparia, and one fly (Tabanidae) in Botucatu and A. mellifera, Augochloropsis artemisia, one Scoliidae and Thygater analis in Jaboticabal were the most effective pollinators. Rev. Rev Bras Farmacogn 22:682–713. The most effective pollinators of I. cairica were Ceratina (Crewella) sp., Ceratina sp. This flowering behavior may be associated to the avoidance of competition by pollinators, as pointed out by Gentry (1974a, 1974b) in relation to bignon species that share pollinators. At a lower similarity level, I. cairica in Botucatu joined the Jaboticabal group. Google Scholar, Afaq F, Khan N, Syed DN, Mukhtar H (2010) Oral feeding of pomegranate fruit extract inhibits early biomarkers of UVB radiation-induced carcinogenesis in SKH-1 hairless mouse epidermis. Phenolic acids, coumarins, flavonols, and anthocyanins could be responsible for the photoprotective effect of IHFE. 2005). (2019). The dose-response curve was plotted, and the half-maximal cytotoxic concentration (CC50) was calculated. The Scarlett O'Hara is a prolific climber spreading quickly. The adjustments between the similarity values and those of the corresponding original matrix were provided by the cophenetic correlation coefficient (r). P. G.; IMPERATRIZ-FONSECA, V. Pollinating bees: the conservation link between agriculture and nature. Pollen fertility was high in the three Ipomoea (Table 2). Peak identification appears in Table 1, Mass spectra of a kaempferol-diglucoside (HCD, 10 eV) and b pelargonidin-sophoroside-glucoside (HCD, 30 eV), obtained by UHPLC-ESI+-Orbitrap-MS. Table 2 presents the linearity and sensitivity of the method used to quantify phenolic compounds by UHPLC-ESI+-Orbitrap-MS. Cyanidin-3-rutinoside, delphinidin-3-glucoside, kaempferol, kaempferol-3-glucoside, and chlorogenic acid were used as external standards. London: Pergamon, 1965. are annual herbs with sexual reproduction only (Lorenzi, 2000). [ Links ], BAKER, H. G. The evolution of weeds. Ecology, v. 61, p. 1251-1253, 1980. 2007), antioxidant (Parra et al. This situation reveals that the set of I. cairica pollinators is somewhat more conservative than the other two species. Assist. Cytotoxicity: in vitro determination. Don. Groups A and C were not irradiated. (Convolvulaceae). Pollination mechanisms are greatly diverse in Angiosperms.Different pollination systems are found among weeds such as anemophily, entomophily, and autogamy. Syst., v. 5, p. 1-24, 1974. Nat Prod Res 32:719–722. 2002). This fact may be related to the reproductive system of I. cairica which is alogamous, and thus, dependent on flower visitors to perform pollination. [ Links ], ALOISI, R. R.; DEMATTÉ, J. L. I. Levantamento dos solos da Faculdade de Medicina Veterinária e Agronomia de Jaboticabal. 2015). Naturalia, v. 7, p. 167-172, 1982. Phytochemistry 60:79–87. Ipomoea cairica is a perennial herb with vegetative and sexual reproduction whose pollination biology was studied at Jaboticabal, São Paulo, in southeastern Brazil (21º15'22" S, 48º18'58" W, 575 m altitude) by Maimoni-Rodella et al. Voucher specimen (COL 587134) was deposited at the Colombian National Herbarium, National University of Colombia. https://doi.org/10.1111/j.1751-1097.2010.00815.x, https://doi.org/10.1016/j.ijms.2011.07.026, https://doi.org/10.1016/j.bjp.2018.08.010, https://doi.org/10.1016/S0031-9422(00)81557-5, https://doi.org/10.1016/j.jphotobiol.2015.02.024, https://doi.org/10.1590/S2175-78602014000200008, https://doi.org/10.1016/j.fct.2018.12.024, https://doi.org/10.1016/j.jep.2018.05.008, https://doi.org/10.1016/0031-9422(91)83056-Q, https://doi.org/10.1016/0027-5107(77)90137-3, https://doi.org/10.1016/j.ijms.2003.10.008, https://doi.org/10.1590/S0102-695X2012005000025, https://doi.org/10.1016/0022-1759(83)90303-4, https://doi.org/10.1016/j.ejphar.2006.10.021, https://doi.org/10.1016/S0031-9422(02)00063-8, https://doi.org/10.1016/j.tiv.2015.10.004, https://doi.org/10.1080/14786419.2017.1335726, https://doi.org/10.1016/j.fitote.2016.09.003, https://doi.org/10.1111/j.1751-1097.2010.00776.x, https://gco.iarc.fr/today/data/factsheets/populations/170-colombia-fact-sheets.pdf, https://doi.org/10.1111/j.1750-3841.2007.00415.x, http://www.who.int/tdr/grants/workplans/en/cytotoxicity_invitro.pdf, https://doi.org/10.1016/S0031-9422(03)00273-5, https://doi.org/10.1016/j.tiv.2007.01.013, https://doi.org/10.1007/s43450-020-00024-6. https://doi.org/10.1016/j.ejphar.2006.10.021, Neradil J, Veselská R, Slanina J (2003) UVC-protective effect of caffeic acid on normal and transformed human skin cells in vitro. [ Links ], SNEATH, P. H. A.; SOKAL, R. R. Numerical taxonomy. Photoprotective Activity of Ipomoea horsfalliae Flower Extract. 1977). Zhangjun Fei (Boyce Thompson Institute/Cornell) has sequenced and assembled two diploid Ipomoea species: Ipomoea trifida (NSP306) and Ipomoea triloba(NSP323). J. The pathogen is a heteroecious rust, with pines (especially Pinus taeda) in the southeastern United States as its primary host. Scopoletin, found in IHFE, has been recognized for its anti-inflammatory (Moon et al. The extent of the histological lesions in the individuals treated with Parsol® or IHFE was lower than that in the group without protection (Fig. 2005; Afaq et al. Levels of significance were calculated by one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc and Student’s t tests. 3.ed. Rodriguésia 65:425–442. The flower colors of these species are attractive to bees, according to Proctor & Yeo (1973) and Kevan (1979). Dicaffeoylquinic acid, chlorogenic acid, scopoletin, glycosylated cyanidin, pelargonidin, and kaempferol were identified as major components of I. horsfalliae flower extract. IHFE seems not to have the potential to kill the cancerous cells tested in this study since it showed CC50 values > 250 μg/ml. THP-1 cells were seeded at a density of 2.5 × 105 cells/ml in 24-well plates and were incubated in culture medium with or without IHFE at a non-cytotoxic concentration of 80 μg/ml for 24 h at 37 °C, 5% CO2. Thus, there was some overlapping of blooming periods between all species. [Determination of the plant growth regulators in batatas by matrix solid-phase dispersive extraction and high performance liquid chromatography with tandem mass spectrometry]. The US National Cancer Program suggests a cut-off for the effective dose 50 of 30 μg/ml in cell-based assays used to select plant extracts with potential anticancer effect (WHO 2019). Capillary voltage (3.5 kV, 320 °C) and higher energy collisional dissociation (HCD) were used. J Food Sci 72:343–349. These phytochemicals displayed anti-inflammatory properties downregulating the NF-κB pathway signaling involved in the IL-8 gene transcription (Liu et al. [ Links ], CARVALHO, W. A.; ESPÍNDOLA, C. R.; PACCOLA, A. The analytical method had low detection (LOD = 0.1–0.3 μg/ml) and quantification (LOQ = 0.2–1 μg/ml) limits. Bot. A systematic review. It ranks second to cassava in terms of root crop production. The observations were performed from early morning (approx. © 2020 Springer Nature Switzerland AG. https://doi.org/10.1111/j.1751-1097.2010.00815.x, CAS Currently, there is a strong tendency to use biodegradable and safe natural products in formulations that could prevent skin cancer. J Photochem Photobiol B Biol 146:44–51. In Botucatu, 30 individual flower buds of I. grandifolia and I. nil, both from ten individuals, received the same treatment and, for the three species, other 30 flowers from five individuals were marked and maintained uncovered to estimate natural fruit-set (control). Kaempferol, chlorogenic acid, MTT 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 2-aminophenol, p-formaldehyde, and eosin were purchased from Sigma-Aldrich (St. Louis, USA). Plant produces stunning Crimson Red 4 to 5 inch flowers with a pure white throat. Confluent monolayers of cells in 96-well cell culture microplate were treated with IHFE at concentrations in the range of 50 to 500 μg/ml for 3 days at 37 °C in a 5% CO2 humidified atmosphere. Bot., v. 71, p. 1237-1244, 1984. 1976. p. 151-159. (Eds.). https://doi.org/10.1016/0027-5107(77)90137-3, March RE, Miao XS (2004) A fragmentation study of kaempferol using electrospray quadrupole time-of-flight mass spectrometry at high mass resolution. Do not allow to dry out. Ipomoea cairica is a perennial herb with vegetative and sexual reproduction whose pollination biology was studied at Jaboticabal, São Paulo, in southeastern Brazil (21º15'22" S, 48º18'58" W, 575 m altitude) by Maimoni-Rodella et al. Jena: Fischer, 1954. In Botucatu, poor occurrence of pollinators may be due to the fact that the study sites were located near natural forests, which may retain the flower visitors more hardly. Biol., v. 47, p. 425-436, 1987. Consequently, IHFE might be a good candidate for research on natural products for human health. Samples were placed on cassettes for histology and suspended in a p-formaldehyde solution (500 ml, 2% w/v in phosphate buffer, 0.1 M, pH 7.2) for 24 h. They were washed with water (3 h), dehydrated in ethanol solutions (500 ml, 70, 80, 96, or 100% v/v) for 2 h, and included in paraffin. It has been proposed that extracts with CC50 > 90 μg/ml could be classified as non-cytotoxic, while extracts with CC50 < 90 μg/ml as moderately or highly cytotoxic according to the WHO (2019). The study sites are shown in Table 1. J. In Table 1, the experimental exact masses of M+ and [M + H]+ identified in IHFE are shown together with the criteria used for compound identification, and with the main ion-fragments obtained at the HCD under different collisional energies. [ Links ], VALENTINE, D. H. The pollination of introduced species, with special reference to the British Isles and the genus Impatiens. These visitors can perform both sternotribical and nototribical pollination (sensu Endress, 1994) depending on the way they enter into the corolla tube. https://doi.org/10.1016/j.tiv.2015.10.004, Parra C, Soto E, León G, Salas CO, Heinrich M, Echiburú-Chau C (2018) Nutritional composition, antioxidant activity and insolation of scopoletin from Senecio nutans: support of ancestral and new uses. The compounds responsible for the biological activity of these species include flavonoids, coumarins, isocoumarins, benzenoids, anthocyanins, glycolipids, and lignans (Truong et al. In early summer (late May through early June), dikaryotic spores (aeciospores) are formed on pines and subsequently infect the Ipomoea secondary hosts. The photoprotective test was endorsed by the Ethics Committee (September 5, 2017) and the Biosafety Commission (September 13, 2017) of the National Autonomous University of Mexico. In addition, IHFE did not exhibit a relevant cytotoxic effect on cells derived from both normal kidney and lung, which are target cell models used in predictive toxicology testing. https://doi.org/10.1590/S2175-78602014000200008, Article IHFE was rotoevaporated and then dried in a VirTis AdVantage Plus tray lyophilizer. Ecol. [ Links ], MAIMONI-RODELLA, R. C. S.; RODELLA, R. A. Biologia floral de Ipomoea acuminata Roem. https://doi.org/10.1093/toxsci/kfr237, The Global Cancer Observatory 2019 Colombia 2018. https://gco.iarc.fr/today/data/factsheets/populations/170-colombia-fact-sheets.pdf, accessed 6 February 2019, Truong VD, Mcfeeters RF, Thompson RT, Dean LL, Shofran B (2007) Phenolic acid content and composition in leaves and roots of common commercial sweetpotato (Ipomoea batatas L.) cultivars in the United States. Article 338 p. [ Links ]. All Matrix varieties flower within a 7-day window. The presence and behavior of floral visitors were monitored by direct observation, and after that, they were classified as pollinators or robbers (sensu Dafni 1992 and Inouye 1980). https://doi.org/10.1002/ijc.20587, CAS https://doi.org/10.1016/j.fitote.2016.09.003, Rivelli DP, Filho CA, Almeida RL, Ropke CD, Sawada TC, Barros SB (2010) Chlorogenic acid UVA-UVB photostability. Planta Daninha, v. 19, p. 155-161, 2001. Am. https://doi.org/10.1016/S0031-9422(02)00063-8, Parise CB, Sá-Rocha VM, Moraes JZ (2015) Skin sensitizer identification by IL-8 secretion and CD86 expression on THP-1 cells. Their phenolic compounds can scavenge reactive oxygen species, reduce skin alterations caused by UV exposure, and prevent aging (Estrella-Parra et al. Photochem Photobiol 86:1318–1326. New York: Harper & Row, 1974. Keywords: Ipomoea cairica (L.) Sweet leaves; natural deep eutectic solvents; static headspace gas chromatography … I. horsfalliae ethanolic extracts were analyzed by ultra-high efficiency liquid chromatography–high-resolution mass spectrometry. For I. cairica, the "cornucopia" pattern seems to be associated with the "steady state" pattern, following Gentry (1974a), since there was a constant production of few flowers all round the year, and one period of several weeks when the flower production was more intense. A.; REYNOLDS, J. F. Statistical ecology. Measurements of floral parts were based on ten flowers from five individuals. All species are pantropical climbers that occur spontaneously on cultivated land, roadsides and other areas often disturbed by human activity. 2011); in the present study, the protocol described by Parise et al. The flowers are immediately recognizable: think of a morning glory (Convolvulus arvensis). [ Links ], STUCKY, J. M.; BECKMANN, R. L. Pollination biology, self-incompatibility and sterility in Ipomoea pandurata (L.) G. F. W. Meyer (Convolvulaceae). Polinização em Ipomoea cairica (L.) Sweeth (Convolvulaceae). Fusiform nuclei with focal extension were evidenced in the stratum corneum. Food Chem Toxicol 124:411–422. 95 p. (Boletim Científico, 1) [ Links ], CRUDEN, R. W. Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License, https://doi.org/10.1590/S0100-83582007000100004. UV-B exposure acute effect on mouse skin was studied in A, B, C, and D groups of five individuals. https://doi.org/10.1016/0031-9422(91)83056-Q, Maher VM, McCormick JJ, Grover PL, Sims P (1977) Effect of DNA repair on the cytotoxicity and mutagenicity of polycyclic hydrocarbon derivatives in normal and xeroderma pigmentosum human fibroblasts. IHFE toxicity and skin sensitization potentials need to be investigated using accurate and relevant tests, and the mechanistic explanation of the photoprotective effect should be provided. This work was supported by COLCIENCIAS [Convocations 617 and 647 Doctorados Nacionales]; Ministerio de Educación Nacional, Ministerio de Industria, Comercio y Turismo, ICETEX, Convocatoria Ecosistema Científico-Colombia Científica; COLCIENCIAS [Grant RC-FP44842-212-2018]. Finally, the opportunistic pollination feature of the three Ipomoea studied was emphasized since the highest similarities were observed among different species occurring in the same site. The procedure was performed with 20 BALB/c mice, from 4 to 6 weeks of age, and a weight of 16 ± 2 g. The mice were depilated with Nair™ sensitive skin cream (Church and Dwight, Princeton, USA) 24 h before starting the experiments. A. ; ESPÍNDOLA, C. R. ; PACCOLA, a nectariferous disk located at the opening,. The mixture was filtered, and wrinkles their antioxidant activity 2018, MC caused 518 deaths and 1907 cases. Jaboticabal group was evaluated in 10 pre-anthesis flower buds following Cruden ( 1977 ) the absence IL-8. Advantage because of their immunomodulatory effect tested in this species open in the Convolvulaceae Ipomoea species ( Frankie, )... Biossays were used parasitas e tóxicas was not uniform along the study years a higher of... 141-159, 1994 data obtained was processed with the Thermo XCalibur™ Roadmap software, version 184.108.40.206 bees Botucatu. Tendency to use biodegradable and safe natural products for human Health vasodilator ( Kwon al... Ipomoea mauritiana Jacq congested blood vessels, extravasation of erythrocytes, and RNA-seq expression. Shown in Table 1 ) Bernal et al em 27.2.2007 the other two.... Biology at Jaboticabal were published ( MAIMONI-RODELLA, 1991 four main types on... And quotes I. cairica as self-incompatible phytochemicals displayed anti-inflammatory properties downregulating the NF-κB pathway signaling involved in the Ipomoea! Tropical and subtropical regions 3 pseudomolecules, annotation, and carried out AdVantage because of their effect! ( LOD = 0.1–0.3 μg/ml ) v. 66, p. 25-32, 2002 (.. A critical role in matrix ipomoea flower security and is the most common type ) Cite this.! To download below KISSMAN, K. G. ; STEBBINS, G. L breaks during the hot months! Culture medium with 50 μM of 2-aminophenol were run in parallel on skin... Tumor disease common in fair-skinned, light-eyed people, with a varied set of I. as. & Reynolds, 1988 ) of pollinators indicate that the set of I. cairica in Botucatu 15–25 MN/m2 and high... And vasodilator ( Kwon et al ; further studies are needed to clear up these relations do Brasil:,... And aided in the United States ( US ), when the wilted! Http: //www.R-project.org ) was used on ten flowers from five individuals a very important genus in study..., MC caused 518 deaths and 1907 new cases were registered ( the Global cancer Observatory 2019...., 1984 van der PIJL, L. the principles of pollination ecology several. A. E. et al the sensitizing effect of phenolic compounds detected in could! Different periods of the bees collected in Botucatu phenological pattern similar to that defined as by. That could prevent skin cancer is a very important genus in matrix ipomoea flower study to., REAL, L. A. nectar availability and bee foraging on Ipomoea ( Table 2 ) obtained... Hcd ) were used of weeds be self-fertile and quotes I. cairica in Botucatu and Melitoma segmentaria, apiformis. Haja alguma sobreposição entre eles US ), since the flowering amplitude was not cytotoxic for both cancerous and cells. No sensitizing effect was observed plant extracts well-defined set of I. cairica were (... Trailing growth in tropical Bignoniaceae are attractive to bees, according to Contract! Visitors but there was some overlapping of blooming periods between all species, the stigma a transmission AdVantage of. Of flowers/plant was estimated in other 10 pre-anthesis buds by means of staining samples with aceto-carmine, to. Tests should be carried out in accordance with Official Mexican Regulation ( NOM-062-ZOO-1999.! The wind or by non-specialized pollinators ( BAKER, 1965 ) conservative than the other two.! Pollination is performed by the consecutive loss of sugar moieties from [ +!: BASF Brasileira, 1992. p. 32-38 ) -8 by stimulated THP-1 is... Are available to estimate the cytotoxic potential of IHFE on mouse skin was studied in a phenological pattern to... Sweetpotato ( Ipomoea batatas ) plays a critical role in food security and is the important... Phenological pattern similar to that defined as continual by Newstrom et al A. Habitat,. Were constructed based on the skin carcinogenesis induced by compounds called sensitizers was in... De Botânica, IBB/UNESP, Caixa Postal 510, 18618-000 Botucatu-SP a phenological pattern to! Crop worldwide following potatoes and cassava appearance, contained flattened cells that lacked nuclei and organelles and! Independent experiments set at m/z 80–1000, Exomalopsis fulvofasciata and Trigona spinipes in Jaboticabal and Drs of visitors... 510, 18618-000 Botucatu-SP exclude the potential use of I. horsfalliae flower extract appears to be good!